Journal: MicrobiologyOpen

Article Title: Synergistic Integration of Recombinase Polymerase Amplification and CRISPR–Cas12a Enables High‐Sensitivity Detection of Mycoplasma bovis

doi: 10.1002/mbo3.70435

Figure Lengend Snippet: Optimization of RPA primers and CRISPR/Cas12a detection for the Mycoplasma bovis uvrC gene. (A) Schematic of p‐uvrC generation by restriction cloning of a 558‐bp uvrC fragment, which was used as the standard DNA template. (B) Screening of eight RPA primer pairs (PP1–PP8) targeting uvrC; PP3–PP8 produced strong, specific amplicons, and target amplification was also detected in DNA from M. bovis ‐positive bison lung. (C) Sequence of three CRISPR guide RNAs (gRNA1–gRNA3) designed within the amplified uvrC region adjacent to 5′‐TTTG‐3′ PAM sites. (D) Comparison of Cas12a fluorescence kinetics showed that all three guides detected uvrC, with gRNA3 producing the fastest and strongest signal. (E) Optimization of ssDNA‐FQ reporter concentration identified 0.75 μM as the best condition for visual detection with minimal background, although maximal fluorescence was reached at 5 μM. (F) All guide RNA combinations produced detectable fluorescence with similar kinetics, although gRNA2 + 3 and gRNA1 + 2 + 3 showed slightly faster onset.

Article Snippet: The target band was excised and purified using the Monarch DNA Gel Extraction Kit (New England Biolabs).

Techniques: CRISPR, Cloning, Screening, Produced, Amplification, Sequencing, Comparison, Fluorescence, Concentration Assay

Journal: iScience

Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

doi: 10.1016/j.isci.2026.117642

Figure Lengend Snippet:

Article Snippet: DNA Plasmid Extraction Kit , Qiagen , Cat No. 27104.

Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software

Journal: iScience

Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease

doi: 10.1016/j.isci.2026.117642

Figure Lengend Snippet:

Article Snippet: Genomic DNA Extraction Kit , New England Biolabs , Cat No. T3010L.

Techniques: Isolation, Cell Isolation, DNA Extraction, Quantitation Assay, Plasmid Preparation, Extraction, Recombinant, Expressing, Software